Leveraging Mass Spectrometry and Atomic Force Microscopy Technologies to Discover & Quantify Hidden Cardiac Fibrosis
Time: 11:30 am
day: Day One
Details:
- Explore how prequalified and validated antibodies are used to visualize ECM proteins and fibrillar collagen through immunoblotting and immunohistochemistry
- Discover the use of second harmonic generation (SHG) microscopy and fluorescence lifetime imaging microscopy (FLIM) for quantifying fibrotic lesions in living rodents
- Learn about the application of LC-MS/MS protocols to quantify distinct forms of collagen, crucial for understanding the mechanisms of fibrosis